Demonstration of liberation of O2 during photosynthesis using Hydrilla Photosynthesis:

 Demonstration of liberation of O2 during photosynthesis using Hydrilla Photosynthesis: It is the reduction of CO2 into carbohydrates (CHO) in presence of chlorophyll and light. Chlorophyll absorbs sunlight and converts it into chemical energy (ATP and NADPH2). Reduction of CO2 by H+ (supplied from H2O) occurs in presence of energy and enzymes present in the green chloroplast. The process takes place in hundred of steps. It can be summarized as follows: Light 6CO2 + 12H2O C6H12O6 + 6H2O + 6O2 Chlorophyll Significance: 1. Neither CO2 nor H2O will burn, nor could they be eaten. Carbohydrate can be eaten and burn easily and release energy slowly. 2. Make usable organic food (carbohydrates) from inorganic substances. 3. Purifies air by absorbing poisonous CO2 and releasing O2. Oxygen is essential for breathing. Existence of life depends on photosynthesis. Objectives of the study:  To know about the liberation of O2 during photosynthesis  To get idea about the rate of photosynthesis in different conditions Materials required: 1) Aquatic plant (Hydrilla) 2) Test tubes 3) Baking soda (NAHCO3) 4) Water (at room temperature) 5) Glass Funnel 6) Knife/Scissors 7) Watch Procedure: 1. A segment of plant of approximately 8cm was cut with scissors. 2. The end of the stem at the site of incision was gently crushed. 3. The plant was covered by a glass funnel under water. 4. A test tube filled with water was inverted over the funnel. 5. The experiment was set in different conditions (Room temperature, sunlight and NAHCO3 solution) 6. Then the formation of air bubbles were observed and counted per time. Observations: Conditions No of air bubbles in 30 seconds No of air bubbles hr-1 Trial 1 Trial 2 Trial 3 Mean Room temp Direct Sunlight NAHCO3 solution Conclusion: Under the given conditions, the plants being tested were able to undergo photosynthesis. Oxygen released by the plants showed that photosynthetic reactions had taken place. Furthermore the amount of oxygen generated indicated the rate of which photosynthesis reactions were occurring. In plants where the light source was of higher (direct sunlight) more oxygen products were found to be produced. In room condition oxygen production was lower than the direct sunlight condition due to less light and temperature. More CO2 was produced in soda solution as a result oxygen production was enhanced in that condition

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